Architecture of metaphase chromosomes and chromosome scaffolds.
نویسندگان
چکیده
منابع مشابه
Architecture of metaphase chromosomes and chromosome scaffolds
We have developed procedures for depositing intact mitotic chromosomes and isolated residual scaffolds on electron microscope grids at controlled and reproducible levels of compaction. The chromosomes were isolated using a recently developed aqueous method. Our study has addressed two different aspects of chromosome structure. First, we present a method for improved visualization of radial chro...
متن کاملNucleosomes in metaphase chromosomes.
Previous studies of the structure of metaphase chromosomes have relied heavily on electron micrography and have revealed the existence of a 10-nm unit fiber that is thought to generate the native 23-30-nm fiber by higher order folding. The structural relationship of these metaphase fibers to the interphase fiber remains obscure. Recent studies on the digestion of interphase chromatin have revea...
متن کاملProteins of metaphase chromosomes and interphase chromatin.
Metaphase chromosomal and interphase chromatin proteins from cells of two species have been compared by polyacrylamide gel electrophoresis. Consistent, common changes in the quantitative distribution of the nonhistone chromosomal proteins are observed in both species. Proteins of ca. 65,000 and 68,000 MW are enriched in interphase chromatin while proteins of ca. 50,000 and 200,000 are more prom...
متن کاملComparison of the proteins present in HeLa cell interphase nucleoskeletons and metaphase chromosome scaffolds.
The nucleoskeleton, a macromolecular complex whose major structural elements are DNA and protein, is purported to be at least in part responsible for the gross morphology of the nucleus. This structure can be isolated from the nuclei of cells in all stages of the cell cycle with the exception of mitosis when no nuclei are present. We had previously proposed that during mitosis, the nucleoskelet...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1983
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.96.1.84